DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples.

Lee, Ji Hyun; Lee, Hye Young; Cho, Sohee; Cho, Joo Youn; Jang, In Jin; Lee, Soong Deok

doi:2013.37.4.220

Korean J Leg Med > Volume 37(4); 2013 > Article

Case Report

Korean Journal of Legal Medicine 2013;37(4):220-223.
DOI: https://doi.org/10.7580/kjlm.2013.37.4.220 Published online November 30, 2013.

DNA Profiling via Short Tandem Repeat Analysis by Using Serum Samples.

Ji Hyun Lee, Hye Young Lee, Sohee Cho, Joo Youn Cho, In Jin Jang, Soong Deok Lee

¹Department of Forensic Medicine, Seoul National University, College of Medicine, Seoul, Korea. sdlee@snu.ac.kr
²Institute of Forensic Science, Seoul National University, College of Medicine, Seoul, Korea.
³Department of Pharmacology, College of Medicine, Seoul National University, Seoul, Korea.

Abstract

Serum is free of cellular components. Because DNA is located in the nuclei or mitochondria of cells, serum could be assumed DNA free. Few previously published case reports to date have used serum for DNA typing. Here, we report on human genotyping via short tandem repeat (STR) analysis using serum as a sample, and discuss problems involved in the process.

Key Words: Serum, DNA Extraction, STR